|
KMID : 0388720100170020049
|
|
Journal of Korean Society of Spine Surgery
2010 Volume.17 No. 2 p.49 ~ p.56
|
|
|
Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy
|
|
Kim Hak-Sun
Lee Kwang-Il
Kim Hyang
Kwon Un-Hye
Nam Mi-Ran
Jang Ju-Woong
Cho In-Je
Kim Bo-Ram
Lee Hwan-Mo
Moon Seong-Hwan
|
|
|
Abstract
|
|
|
Study Design: This is an in-vitro experiment using rabbit intervertebral disc (IVD) cells and growth factors.
Objectives: We wanted to determine the effect of types I,and II atelocollagen and growth factor gene therapy for matrix regeneration of rabbit IVD cells.
Summary of the Literature Review: Adenovirus-medicated growth factor gene therapy is efficient for matrix regeneration of the IVD. Atellocollagen has provided a favorable environment for matrix synthesis. However, a combined approach using gene and cell therapy in an atelocollagen scaffold has not yet been attempted.
Materials and Methods: Rabbit IVD cells were transduced with Ad/TGF-β1 and Ad/BMP-2. The cells were then implanted to the atelocollagen scaffold. The [methyl-3H]thymidine incorporation for DNA synthesis and the [35S]sulfur incorporation for proteoglycan synthesis were measured. RT-PCR was performed for assessing the aggrecan, collagen type I, collagen type II and osteocalcin mRNA expressions.
Results: The rabbit IVD cells with Ad/TGF-β1 and that were cultured in type I atelocollagen showed a 130% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/TGF-β1 and that were cultured in type II atelocollagen showed a 180% increase in new proteoglycan synthesis (p<0.05). The rabbit IVD cells with Ad/BMP-2 and that were cultured in type I atelocollagen showed a 70% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/BMP-2 and that were cultured in type II atelocollagen showed a 95% increase (p<0.05). Rabbit IVD cells with Ad/TGF-β1 and Ad/BMP-2 and that were cultured in type I and II atelocollagen demonstrated increased collagen type I and II mRNA expressions without an osteocalcin mRNA expression (p<0.05).
Conclusion: Cell and gene therapy in an atelocollagen scaffold provided a efficient mechanism for chondrogenic matrix regeneration of rabbit IVD cells.
|
|
|
KEYWORD
|
|
|
Intervertebral disc, Collagen, Scaffold, TGF-β1, BMP-2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|